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MANUSCRIPT FORMATTING REQUIREMENTS

1.- Introduction
Journal of Ozone Therapy (JO3T) borns from the necessity of having a common place to publish scientific papers about the medical uses of ozone and its fundamentals that enhance the interchange of knowledge on these topics inside the scientific community.

2.- Official Language
As official journal of the World Federation on Ozone Therapy (WFOT), all articles should be sent in English language. We advice the authors to have a revision by a native English speaking colleague before sending the paper to the JO3T.
All articles can also be published in the native language of the authors as an addition to the official publication, if it is different from English. Authors must send a copy of the article to JO3T in the native language in a PDF file, including text, tables, images and all parts in a proper layout that will not be altered by the Production Editor.

3.- Article sections

Articles published in JO3T should fit one of the following formats (sections):

a) Original Articles.
b) Review Articles.
c) Opinion Articles.
d) Case Reports.
e) Proceedings/Meeting abstracts.
f) Letters to the Editor.
g) Editorial.

Please read the Section Policies for detailed information.

4.- Parts of the manuscript:

4.1. Previous information
a) With each new manuscript submission, authors must select the Category of the article: Original Article, Case Report, Review, etc.
b) The authors can send "Comments for the editor" describing the significance of the work, its uniqueness, and any similar work the authors reported previously.

4.2. Submission Checklist
Indicate that this submission is ready to be considered by this journal by checking off the following.
a) Full title of the article.
b) Name, address, telephone, and e-mail address of the corresponding author.
c) Names of each author’s institution and an indication of each author’s affiliation.
d) Acknowledgments of research support for the study. The Acknowledgments section lists all funding sources for the research of the study, and details substantive contributions of individuals.
e) Disclosure of potential conflicts of interest. The authors must reveal all possible Conflicts of Interest/Disclosures.
f) List of where and when the study has been presented in part elsewhere, if applicable.
g) Appropriate keywords should be selected from the Medical Subject Heading.
h) Intellectual property permissions. Instructions are published in our Intellectual Property page.

4.3. Abstract
- Limit the abstract to 350 words
- Do not use proprietary or trade names in the title or abstract.
- Editorials and Letters to editors do not require abstracts.

Format the abstract according to the following headings:

FOR ORIGINAL ARTICLES:
- Purpose
- Patients and methods (or Materials and Methods, Methods, or similar heading). In abstracts of Review Articles, authors may use Design instead of Patients and methods)
- Results
- Discussion
- Conclusion

FOR REVIEW ARTICLES:
- Purpose
- Design (Optional)
- Results
- Discussion
- Conclusion

FOR OPINION ARTICLES:
- Introduction
- Argumentation
- Conclusion

FOR CASE REPORTS:
- Introduction
- Literature review
- Case Presentation
- Discussion
- Conclusion

4.4. Text Pages
Write the body of the manuscript as concisely as possible.
Adhere to the manuscript category word limits described here in.
Number and double space all pages.
Use a common font such as Helvetica or Arial fonts at 12-point size.
Adhere to the style guidelines set forth by the ICMJE (International Committee of Medical Journal Editors).

Organize the manuscript into several headings depending the article category:

FOR ORIGINAL ARTICLES:
a) Introduction   
b) Patients and Methods (or Materials and Methods)
c) Results
d) Discussion
e) Conclusion
f) References

FOR REVIEW ARTICLES:
a) Purpose
b) Design (Optional)
c) Results
d) Discussion
e) Conclusion
f) References

FOR OPINION ARTICLES:
a) Introdcution
b) Argumentation
c) Conclusion
d) References

FOR CASE REPORTS:
a) Introduction
b) Literature review
c) Case Presentation
d) Discussion
e) Conclusion
f) References

5.- Abbreviations
- Define abbreviations at first mention in text and in each table and figure.
- For a list of standard abbreviations, consult the Council of Biology Editors Style Guide (available from the Council of Science Editors, 9650 Rockville Pike, Bethesda, MD 20814, www.councilscienceeditors.org) or other standard sources.
- Write out the full term for each abbreviation at its first use unless it is a standard unit of measure.

6.- Brand name mentions
- If a brand name is cited, supply the manufacturer’s name and address (city and state/country).
- The complete names and short addresses of manufacturers of any equipment used in Materials and Methods must be supplied.

7.- References (Vancouver Style)
- Double space and list references after the body text under the heading: REFERENCES.
- Number references in the order in which they are cited in the text.
- Conform abbreviations of medical periodicals, if suitable, to those in the “List of Journals Indexed in Index Medicus” in the latest edition of Index Medicus and on MEDLINE.
- Limit of references has been detailed in “Article categories” section.
- When a reference is an abstract or supplement, it must be identified as such in parentheses at the end of the reference.
- Abstract and supplement numbers should be provided, if applicable.
- When a reference is unpublished data, a manuscript in preparation, or a manuscript submitted but not in press, it should be included in parentheses in the body of the text, and not cited in the reference list.
- Personal communications should also be listed parenthetically, and should contain the first initial and last name of the contact, as well as the month and year of the communication.
- Published manuscripts, and those that have been accepted and are pending publication, should be cited in the reference list.

Reference Style (Vancouver), Examples:

Journal article with one, two, or up to six authors:
1. Dolan ME, Pegg AE. O6-Benzylguanine and its role in chemotherapy. Clin Cancer Res. 1987;8:837-847.
 
Journal article with more than six authors:
2. Diaz MT, Fraga A, Dranguet J, Mallok A, Viebahn R, Menendez S, et al. Ozone oxidative postconditioning protects against the injury associated with alcohol withdrawal syndrome in rats. Ozone Sci Eng. 2012;34(6):425-431.
 
Journal article in press (manuscript has been accepted for publication)
3. Scadden DT, Schenkein DP, Bernstein Z. Combined immunotoxin and chemotherapy for AIDS-related non-Hodgkin’s lymphoma. Cancer (in press).

Journal article not in English language:
4. Kleinsteuber-Saa K, Avaria-Benapres M, Varela-Estrada X. Actualizacion en paralisis cerebral infantil [Update in child
cerebral palsy]. Rev Pediatr. 2014;11(2):1-3.

Supplement:
5. Brusamolino E, Orlandi E. Analysis of long- term results and prognostic factors among 138 patients with advanced Hodgkin’s disease treated with the alternating MOPP/ABVD chemotherapy. Ann Oncol. 1994;5(suppl 2):S53-S57.
 
Book with a single author:
6. Woodruff R: Symptom Control in Advanced Cancer. Victoria, Australia:Asperula Pty Ltd; 1997.
 
Book with multiple authors:
7. Iverson C, Flanagin A, Fontanarosa PB. American Medical Association Manual of Style. 9th ed. Baltimore (MD): Williams & Wilkins; 1998.
 
Chapter in a multiauthored book with editors:
8. Seykora JT, Elder DE. Common acquired nevi and dysplastic nevi as precursor lesions and risk markers of melanoma. In: Kirkwood JM. Molecular Diagnosis and Treatment of Melanoma. New York (NY): Marcel Dekker; 1998.
 
Abstract:
9. Bardia A, Wang AH, Hartmann LC. Physical activity and risk of postmenopausal breast cancer defined by hormone receptor status and histology: A large prospective cohort study with 18 years of follow up [abstract] .J Clin Oncol. 2006;24:49.
 
Conference/meeting presentation:
10. Furton EJ, Dort V. Addiction and compulsive behaviors. Proceedings of the 17th Workshop for Bishops; 1999; Dallas, TX. Boston: National Catholic Bioethics Center (US); 2000.
 
Internet resource:
11.  AMA: helping doctors help patients [Internet]. Chicago: American Medical Association; c1995-2007 [cited 2007 Feb 22]. Available from: http://www.ama-assn.org/.

Digital Object Identifier (DOI)
12. Small EJ, Smith MR, Seaman JJ. Combined analysis of two multicenter, randomized, placebo-controlled studies of pamidronate disodium for the palliation of bone pain in men with metastatic prostate cancer. J Clin Oncol. 2003;22(1):122-124. doi: 10.1200/JCO.2003.05.147.

For other situations, please visit NLM web site 

8.- Figures

8.1.- Figure files
If you are submitting a new manuscript, embed each figure in the manuscript in read order, immediately following the paragraph where the figure is first mentioned and above the related figure caption.
Upon revision, each figure must be prepared and submitted to the submission system as an individual file. Additionally, embed the same figures in the manuscript in read order, and ensure that they match the figure files that you uploaded.
Upon acceptance, figure files should be uploaded to the submission system, with no embedded figures in the manuscript.

8.2.- Figure citations
Cite figures in ascending numeric order upon first appearance in the manuscript file.
All images must be sent in digital format, with resolution of at least 900 pixels wide. Authors should submit figures at 100% size whenever possible.
Cite figures in the order they appear in the text using Arabic numerals.
Images may be embedded in PowerPoint files.

8.3.- Accepted and Not accepted formats of figures at first submission

8.3.1.- Accepted formats are as follows:
.jpeg
.jpg
.png
.pdf (PDF)
.ppt / .pptx (Power Point)

8.3.2.- Not accepted formats are as follows:
Any file using OLE (Object Linking and Embedding) technology to display information or embed files
Bitmap (.bmp)
PICT (.pict)
Excel (.xls)
Photoshop (.psd)
Canvas (.cnv)
CorelDRAW (.cdr)
Illustrator (.ai)
Locked or encrypted PDFs

8.4.- Figure Legends
All figures must have a unique and descriptive legend.
Define all relevant and explanatory information extraneous to the actual figure, including figure part labels, footnotes, abbreviations, acronyms, arrows, and levels of magnification in insets.
Double space legend.
Concise as possible.

8.5.- Figures After Acceptance
Submit all line graphs, bar graphs, flow charts, scatter plots, forest plots and diagrams as vector-based artwork, not bitmapped art (ie, no .bmp, .tiff, .gif, or .jpeg files) with a resolution of at least 1200 dpi.
Submit vector artwork from common applications: Adobe Illustrator, Microsoft Word, PowerPoint, or Excel are usable.
For typeface within figures, use 6 pt to 12 pt Universe (Arial or Helvetica are also acceptable). Eps files with fonts embedded (not converted to paths) are preferred.
Submit photographs (halftones) with a minimum resolution of 300 dpi at final size (100% scale).
Do not embed figure numbers or letters in figure parts of photographs.
Place labels on top of the image or set outside of each part. Labels may also be listed in figure legends.
Upon acceptance, a graphic artist will format all figures to JO3T style.

9.- Tables
Use the following rules when creating your tables:
Cite tables in the order in which they appear in the text using Arabic numerals.
Tables must be numbered in the order cited in the text.
Editable tables (i.e., not a graphic object)
Cell-based structure (e.g., create tables in Word or Power Point with the Table tool (or if necessary, Excel))
Row and column organization, not structured with returns, spaces, or tabs
Place each table in your manuscript file directly after the paragraph in which it is first cited (read order). Do not submit your tables in separate files.
Tables require a label (e.g., “Table 1”) and brief descriptive title to be placed above the table.
Use type of the same font and size as employed in the text.
Include footnotes at the bottom of each table. The legend should include any pertinent notes and must include definitions of all abbreviations and acronyms used in the table.
Tables should not duplicate data given in the text or figures.

Please note that JO3T does not allow table pieces, such as Table 1a and 1b. Manuscripts will be returned for correction if they include table pieces.

10. Videos
Where appropriate, video sequences may be submitted using standard digital video formats that would be used by most readers.
Videos should be short (less than 30 seconds) and only contain relevant information.
A description of each video should be provided in the article text.
Acceptable file formats include QuickTime non-streaming video (.qt or .mov), MPEG (.mpg).
The preferred formats are .mov and .mpg.
All videos should be uploaded during the submission process as supplemental files.
If accepted, the videos will be published as data supplements available online.

11. Acknowledgments
Acknowledgments should include any contributors who did not meet the requirements for authorship and any science writers or corporate employees who participated in the development of the manuscript.The Acknowledgments section should appear on your submitted manuscript file after the body text and before the references.

12. Appendices/Supplemental Material
The supplemental data section should only be used for content that is directly relevant to the submitted manuscript, but which could not be included due to space limitations.
New data that is not directly relevant to the manuscript should be submitted as an independent paper subject to separate peer review.